Agrobacterium rhizogenes Mediated Transformation of the Critically Endangered Species, Swertia chirayita
نویسندگان
چکیده
منابع مشابه
Efficient Regeneration and Antioxidant Activity of the Endangered Species Swertia Chirayita Vijay Kumar and Sheela Chandra
An efficient in vitro regeneration protocol was established for Swertia chirayita. The present study describes the regeneration of S. chirayita from shoot tip explants collected from wild grown plantlets. The highest number of shoots (26.10 ± 0.73) were obtained on Murashige and Skoog basal medium supplemented with 6-benzyladenine (BA) (0.5mg/l) in combination with gibberellic acid (GA3) (1.0 m...
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BACKGROUND Plants within the Orobanchaceae are an agriculturally important group of parasites that attack economically important crops to obtain water and nutrients from their hosts. Despite their agricultural importance, molecular mechanisms of the parasitism are poorly understood. METHODOLOGY/PRINCIPAL FINDINGS We developed transient and stable transformation systems for Phtheirospermum jap...
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Using Agrobacterium rhizogenes due to create hairy roots is a useful method to product secondary metabolites in many medicinal plants. The transgenic hairy roots were induced from Peganum multisectum (Maxim) Bobrov a medicinally important species, by infecting leaf and stem explant with wild type Agrobacterium rhizogenes strain ATTCC 15834, which led to the induction of hairy roots from 19% of ...
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To improve Agrobacterium-mediated transformation of tobacco, factors influencing gene delivery, including genotype of the plant, bacterial strain, and Agrobacterium transformation procedure, were tested via direct somatic embryogenesis. Leaf tissue of three different tobacco genotypes (Nicotiana tabacum L. cvs. Samsun, and Xanthi, and N. benthamiana) we...
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ژورنال
عنوان ژورنال: Plant Tissue Culture and Biotechnology
سال: 2019
ISSN: 1818-8745,1817-3721
DOI: 10.3329/ptcb.v29i2.44512